Seminar on Fundamentals of Mass spectrometry for Biomedical Research
Date: March 9 - 10, 2014
Timings: 9:00 AM Onwards
Venue: Institute of Bioinformatics
| Tentative Seminar Program |
Day 1:
Lecture series and Panel discussion
| Time |
Title |
Speaker |
| 09:00 - 09:45 AM |
Evolution of mass spectrometry |
Santosh Renuse
Institute of Bioinformatics, Bangalore |
| 09:45 - 10:30 AM |
Introduction to proteomics and Human Proteome map |
Prof. Akhilesh Pandey
Johns Hopkins University, Baltimore |
| 10:30 - 11:00 AM |
Tea break |
| 11:00 - 11:45 AM |
Proteomics and phosphoproteomic approaches to investigate cancer biomarkers and therapeutics |
Dr. Aditi Chatterjee
Institute of Bioinformatics, Bangalore |
| 11:45 - 12:30 PM |
Proteomics of eye: a platform for molecular investigations of common eye diseases |
Dr. Krishna R. Murthy
Vittala International Institute of Opthalmology, Bangalore |
| 12:30 - 01:30 PM |
Lunch break |
| 01:30 - 02:15 PM |
Proteogenomics: a novel approach to enrich genome annotation |
Dr. Keshava Prasad
Institute of Bioinformatics, Bangalore |
| 02:15 - 03:00 PM |
Multi-omic approaches to study glioblastoma |
Dr. Ravi Sirdeshmukh
Institute of Bioinformatics, Bangalore |
| 03:00 - 03:30 PM |
Tea break |
03:30 - 05:30 PM |
Panel discussion |
Prof. Akhilesh Pandey
Johns Hopkins University, Baltimore
Dr. Ravi Sirdeshmukh
Institute of Bioinformatics, Bangalore
Dr. Harsha Gowda
Scripps Center for Metabolomics and Mass Spectrometry, California
Dr. S. K. Shankar
National Institute of Mental Health and Neurosciences, Bangalore
Dr. Krishna R. Murthy
Vittala Internation Institute of Opthalmology, Bangalore |
|
Day 2:
Hands-on training and demonstration
Hands-on training
Sample preparation
- peptide extraction and iTRAQ labeling
- In-gel and in-solution digestion
Mass spectrometry data analysis
- Analysis of MS/MS data
- Analysis of quantitative proteomic data
Demonstration
Operation of HPLC
- Fractionation: depletion strategies
- Fractionation: Strong Cation Exchange chromatography
- Fractionation: Basic pH Reverse Phase Liquid chromatography
Enrichment of phosphopeptide
Operation of LTQ-Orbitrap Velos mass spectrometer
Analysis of phosphoproteomic data
Biological interpretation of proteomics data |
|