DBT Programme Support on Neuroproteomics for Proteomic Investigation of Neurological Disorders

This multi-institutional collaborative research grant is provided by DBT, Government of India to IOB and NIMHANS to set up the genomic and proteomic platform to investigate potential biomarkers in neurological diseases. The state of art proteomic and computational facilities have been established at IOB from this grant. The major goal of this research grant is to investigate candidate protein biomarkers for 'chronic meningitis' and 'neurological stroke' through high-throughput and high-resolution technologies including DNA microarrays and quantitative proteomics.

CSIR grant : "Genomic and proteomic profiling of gallbladder cancer"

Gallbladder cancer is a leading cancer in terms of mortality rates, which is largely due to lack of early detection. Objective of this research project is to discover biomarkers for early detection of gallbladder cancer. We are carrying out genome-wide expression profiling in gallbladder tumors using DNA microarrays to identify genes that are upregulated in gallbladder cancers. In addition, we will also carry out proteomic profiling using quantitative and high resolution mass spectrometry.  Finally, we will validate potential biomarkers using immunohistochemical labeling of tissue microarrays.

DBT multi-institutional coordinated research grant: “Establishment of National Database on Tuberculosis (TB)”

India accounts for about 30% of the global burden of tuberculosis - more than any other country in the world.   The objectives of this research project include development of a Proteomic and Host-Pathogen Interaction Database for tuberculosis.  We have also developed a central website for integrating data from ‘TBNET India programme’ conceived by DBT. It will also serve as a binding force for the various arms of the ‘TBNET India’. 

DBT multi-institutional research grant: “Identification of genes involved in X-linked mental retardation”

Identification of precise molecular mechanisms involved in X-linked mental retardation will lead to better classification, diagnosis, prevention and therapy.  This research project aims to identify genes and genetic mechanisms that may play a role in these disorders using custom designed microarrays for comparative genomic hybridization.  

DBT research grant “Mouse Protein Reference Database”

In the last several decades, vast amounts of genetic, genomic and proteomic data derived from systematic experimentation in mice have become available. Aim of this project is to develop a Mouse Protein Reference Database (MPRD) as a comprehensive repository of information pertaining to mouse proteins including domain architecture, protein functions, protein-protein interactions, post-translational modifications, enzyme-substrate relationships, sub-cellular localization and tissue expression.  It will also include a mouse knockout database as well as data on mouse models of human disease.

DBT Young Investigator grant to a Faculty Scientist, Dr. Keshava Prasad: “A functional analysis of Protein Tyrosine Phosphatases in EGF Receptor signal transduction pathway using a novel Substrate Trapping Mutant strategy”

In this proposal, we investigate the role of tyrosine phosphatases involved with receptor kinases signaling pathways by using a variety of functional approaches such as substrate trapping mutants and yeast two hybrid analysis.

ICMR Senior Research Fellow grant to a Research Scientist, Manoj Kashyap
Research Title: “mRNA and genome copy number profiling of esophageal cancers”

Esophageal cancer is the third leading cancer in males and fourth leading in females in India, but not much is known about the molecular etiology of the disease. Using DNA microarray technology, we obtain gene expression profiles in esophageal cancer. Differentially expressed molecules are being validated using immunohistochemistry and tissue microarrays. These studies will provide candidate biomarkers for early detection of esophageal cancers.

CSIR Senior Research Fellow grant to a Research Scientist, Harrys Kishore
Research Title: “Functional analysis of Hematopoetic SH2 domain containing protein 2D (HSH2D) in B cell Receptor (BCR) Signaling”

Role of HSH2D an SH2 domain containing protein in the BCR pathway. Objective of this project is to identify substrates of HSH2D in BCR signaling.

CSIR Senior Research Fellow grant to a Research Scientist, Jyoti Sharma
Research Title: “Genome-wide analysis of kinome and phosphatome of mycobacteria”

The main focus of this study is to tyrosine kinases and phosphatases in mycobacterial genomes. Results of this analysis will be incorporated into the Mycobacterium tuberculosis Reference Database.